The University of Western Australia

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Joshua Mylne

Dr Joshua Mylne

Future Fellow
Chemistry and Biochemistry, School of

Contact details
Address
School of Chemistry and Biochemistry
The University of Western Australia (M310)
35 Stirling Highway
CRAWLEY WA 6009
Australia
Phone
+61 8 6488 4415
Fax
+61 8 6488 4446
Email
joshua.mylne@uwa.edu.au
Personal homepage
http://www.mylne.org
Qualifications
BSc PhD Qld
Biography
Dr Mylne (PhD, Botany) worked at the John Innes Centre (2001-2005), using molecular genetics to study proteins that accelerate flowering in response to prolonged cold (vernalization). In 2006 he moved to the Division of Chemistry & Structural Biology at The Institute for Molecular Bioscience (IMB, UQ) where he held a QEII Fellowship (2008-2012) and was the inaugural John S. Mattick Fellow (2010-2012). In 2013 he joined the faculty at The University of Western Australia and took up an ARC Future Fellowship in the School of Chemistry & Biochemistry and The ARC Centre of Excellence in Plant Energy Biology. His research interests are protein evolution, molecular mechanisms underlying the biosynthesis of bioactive peptides and increasing the discovery of novel herbicides and novel modes of action.
Key research
Plant peptide biosynthesis
Protease evolution
Herbicides
Publications
Franke, Colgrave, Mylne, Rosengren (2016) Mature forms of the major seed storage albumins in

sunflower: a mass spectrometric approach Journal of Proteomics on-line: TBA-TBA.
href="http://www.ncbi.nlm.nih.gov/pubmed/27185550" target="_blank">PubMed
,
href="http://dx.doi.org/10.1016/j.jprot.2016.05.004" target="_blank">Publisher
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Evans-Roberts, Mitchenall, Wall, Leroux, Mylne, Maxwell (2016) DNA gyrase is the target for the

quinolone drug ciprofloxacin in Arabidopsis thaliana J. Biol. Chem. 291: 3136-3144.

PubMed,
href="http://dx.doi.org/10.1074/jbc.M115.689554" target="_blank">Publisher
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Jayasena, Franke, Rosengren, Mylne (2016) A tripartite approach identifies the major sunflower

seed albumins Theoretical and Applied Genetics 129: 613-629.
href="http://www.ncbi.nlm.nih.gov/pubmed/26767835" target="_blank">PubMed
,
href="http://dx.doi.org/10.1007/s00122-015-2653-3" target="_blank">Publisher
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Bernath-Levin, Nelson, Elliott, Jayasena, Millar, Craik, Mylne (2015) Peptide macrocyclization by

a bifunctional endoprotease Chemistry & Biology 22: 571–582.
href="http://www.ncbi.nlm.nih.gov/pubmed/25960260" target="_blank">PubMed
,
href="http://dx.doi.org/10.1016/j.chembiol.2015.04.010" target="_blank">Publisher
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Gandy, Corral, Mylne*, Stubbs* (2015) An interactive database to explore herbicide physicochemical

properties. Organic & Biomolecular Chemistry 13: 5586-5590. (*co-corresponding)
href="http://www.ncbi.nlm.nih.gov/pubmed/25895669" target="_blank">PubMed
,
href="http://dx.doi.org/10.1039/C5OB00469A" target="_blank">Publisher
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Mahatmanto, Mylne, Poth, Swedberg, Kaas, Schaefer, Craik (2015) The evolution of Momordica

cyclic peptides. Molecular Biology and Evolution 32: 392-405.
href="http://www.ncbi.nlm.nih.gov/pubmed/25376175" target="_blank">PubMed
,
href="http://dx.doi.org/10.1093/molbev/msu307" target="_blank">Publisher
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Jayasena, Secco, Berkowitz, Bernath-Levin, Whelan, Mylne (2014) Next generation sequencing and

de novo transcriptomics to study gene evolution. Plant Methods 10: 34.
href="http://www.ncbi.nlm.nih.gov/pubmed/25364374" target="_blank">PubMed
,
href="http://dx.doi.org/10.1186/1746-4811-10-34" target="_blank">Publisher
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Elliott, Delay, Liu, Phua, Rosengren, Benfield, Panero, Colgrave, Jayasena, Dunse, Anderson, Schilling,

Ortiz-Barrientos, Craik, Mylne (2014) Evolutionary origins of a bioactive peptide buried within

preproalbumin. Plant Cell 26: 981-995.
href="http://www.ncbi.nlm.nih.gov/pubmed/24681618" target="_blank">PubMed
,
href="http://www.plantcell.org/cgi/content/short/tpc.114.123620?keytype=ref&ijkey=nHTFlksp0FtA5F1"

target="_blank">Publisher
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Mylne, Hara-Nishimura, Rosengren (2014) Seed storage albumins: biosynthesis, trafficking and

structures (Goldacre Review). Functional Plant Biology on-line early.
href="http://dx.doi.org/10.1071/FP14035" target="_blank">Publisher
.

Mahatmanto, Poth, Mylne, Craik (2014) A comparative study of extraction methods reveals preferred

solvents for cystine-knot peptide isolation from Momordica cochinchinensis seeds.

Fitoterapia 95: 22-33.
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Rosa*, De Lucia*, Mylne*, Zhu*, Ohmido, Pendle, Kato, Shaw, Dean (2013) Physical clustering of

FLC alleles during Polycomb-mediated epigenetic silencing in vernalization. Genes &

Development
27: 1845-1850. (*equal)
target="_blank">PubMed
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King, Chanson, McCallum, Ohme-Takagi, Byriel, Hill, Martin, Mylne (2013) The Arabidopsis B3

domain protein VERNALIZATION1 is involved in processes essential for development with structural and

mutational studies revealing its DNA binding surface. J. Biol. Chem. 288: 3198–3207.
href="http://www.ncbi.nlm.nih.gov/pubmed/23255593" target="_blank">PubMed
,
href="http://www.jbc.org/cgi/doi/10.1074/jbc.M112.438572" target="_blank">Publisher
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Mylne, Chan, Chanson, Daly, Schaefer, Bailey, Nguyencong, Cascales, Craik (2012) Cyclic peptides

arising by evolutionary parallelism via asparaginyl-endopeptidase mediated biosynthesis. Plant

Cell
24: 2765-2778.
target="_blank">PubMed
,
target="_blank">Publisher
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Poth, Mylne, Grassl, Lyons, Millar, Colgrave, Craik (2012) Cyclotides associate with leaf

vasculature and are the products of a novel precursor in Petunia (Solanaceae). J. Biol. Chem.

287: 27033-27046.
target="_blank">PubMed
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Craik, Henriques, Mylne, Wang (2012) Cyclotide isolation, characterization and bioassay. in

Natural product biosynthesis by microorganisms and plants (3rd edition) Methods in Enzymology

516: 37-62. PubMed,
href="http://dx.doi.org/10.1016/B978-0-12-394291-3.00024-1" target="_blank">Publisher
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Craik, Swedberg, Mylne, Cemazar (2012) Cyclotides as a basis for drug design. Expert Opinion on

Drug Discovery
7:179-194.
target="_blank">PubMed
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target="_blank">Publisher
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Mylne, Mas, Hill (2012) NMR assignment and secondary structure of the C-terminal DNA binding

domain of Arabidopsis thaliana VERNALIZATION1. Biomolecular NMR Assignments 6: 5-8.

PubMed,
href="http://dx.doi.org/10.1007/s12104-011-9313-6" target="_blank">Publisher
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Mohimani, Liu, Mylne, Poth, Colgrave, Tran, Selsted, Dorrestein, Pevzner (2011) Cycloquest:

Identification of cyclopeptides via database search of their mass spectra against genome databases.

Journal of Proteome Research 10: 4505-4512.
href="http://www.ncbi.nlm.nih.gov/pubmed/21851130" target="_blank">PubMed
,
href="http://dx.doi.org/10.1021/pr200323a" target="_blank">Publisher
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Mylne & Wigge (2011) Florigen takes two to tango. Nature Chemical Biology 7:665-666.

PubMed,
href="http://www.nature.com/nchembio/journal/v7/n10/full/nchembio.666.html"

target="_blank">Publisher
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Elliott, Craik, Mylne (2011) Sunflower trypsin inhibitor-1: chemical v. biological synthesis.

Proceedings of the 22nd American Peptide Symposium, ed. Michal Lebl, Prompt Scientific

Publishing, San Diego, CA, pp86-87.
target="_blank">PDF
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Mylne, Colgrave, Daly, Chanson, McCallum, Jones, Elliott, Craik (2011) Albumins and their

processing machinery are hijacked for cyclic peptides in sunflower. Nature Chemical Biology

7: 257-259. PubMed,
href="http://dx.doi.org/10.1038/NChemBio.542" target="_blank">Publisher
.

Box, Coustham, Dean, Mylne (2011) Protocol: A simple phenol-based method for 96-well extraction of

high quality RNA from Arabidopsis. Plant Methods 7: 7.
href="http://www.ncbi.nlm.nih.gov/pubmed/21396125" target="_blank">PubMed
,
href="http://www.plantmethods.com/content/7/1/7" target="_blank">Publisher
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Mylne, Wang, van der Weerden, Craik (2010). Cyclotides are a component of the innate defence of

Oldenlandia affinis. Biopolymers (Pept. Sci.) 94: 635-646.
href="http://www.ncbi.nlm.nih.gov/pubmed/20564018" target="_blank">PubMed
,
href="http://dx.doi.org/10.1002/bip.21419" target="_blank">Publisher
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Qin, McCallum, Kaas, Saska, Craik, Mylne (2010). Identification of candidates for cyclotide

biosynthesis and cyclization by expressed sequence tag analysis of Oldenlandia affinis. BMC

Genomics
11: 111.
target="_blank">PubMed
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target="_blank">Publisher
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Craik, Mylne, Daly (2010). Cyclotides: macrocyclic peptides with applications in drug design and

agriculture. Cell. Mol. Life Sci. 67: 9-16.
href="http://www.ncbi.nlm.nih.gov/pubmed/19795188" target="_blank">PubMed
,
href="http://dx.doi.org/10.1007/s00018-009-0159-3" target="_blank">Publisher
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Trabi, Mylne, Sando, Craik (2009). Circular proteins from Melicytus (Violaceae) refine the

conserved protein and gene architecture of cyclotides. Organic & Biomolecular Chemistry. 7:

2378 – 2388. PubMed,
href="http://dx.doi.org/10.1039/b823020j" target="_blank">Publisher
.

King, Hill, Martin, Mylne (2009). Expression, purification and preliminary X-ray diffraction

studies of VERNALIZATION1208-341 from Arabidopsis thaliana. Acta Crystallogr. F

65: 291-294. PubMed,
href="http://scripts.iucr.org/cgi-bin/paper?S1744309109004217" target="_blank">Publisher
.

Pien, Fleury, Mylne, Crevillen, Inzé, Avramova, Dean, Grossniklaus (2008). ARABIDOPSIS TRITHORAX1

dynamically regulates FLOWERING LOCUS C activation via histone 3 lysine 4 trimethylation. Plant

Cell
20: 580-588.
target="_blank">PubMed
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target="_blank">Publisher
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Mylne & Craik (2008). 15N cyclotides by whole plant labeling. Biopolymers (Pept. Sci.)

90: 575-580. PubMed,
href="http://dx.doi.org/10.1002/bip.21012" target="_blank">Publisher
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Herrmann, Burman, Mylne, Karlsson, Gullbo, Craik, Clark, Göransson (2008). The alpine violet,

Viola biflora, is a rich source of novel cyclotides with potent cytotoxicity.

Phytochemistry 69: 939-952.
target="_blank">PubMed
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target="_blank">Publisher
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Greb*, Mylne*, Crevillen, Geraldo, An, Gendall, Dean (2007). The PHD finger protein VRN5 functions

in the epigenetic silencing of Arabidopsis FLC. Current Biology 17: 73-78. (*equal) .
href="http://www.ncbi.nlm.nih.gov/pubmed/17174094" target="_blank">PubMed
,
href="http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02570-X" target="_blank">Publisher
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Mulvenna, Mylne, Bharathi, Burton, Shirley, Fincher, Anderson, Craik (2006). Discovery of

cyclotide-like protein sequences in graminaceous crop plants: ancestral precursors of circular proteins?

Plant Cell 18: 2134-2144.
target="_blank">PubMed
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target="_blank">Publisher
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Mylne, Barrett, Tessadori, Mesnage, Johnson, Bernatavichute, Jacobsen, Fransz, Dean (2006). LHP1,

the Arabidopsis homologue of HETEROCHROMATIN PROTEIN1 is required for epigenetic silencing of FLC.

Proc. Natl Acad. Sci. U.S.A. 103: 5012-5017.
href="http://www.ncbi.nlm.nih.gov/pubmed/16549797" target="_blank">PubMed
,
href="http://www.pnas.org/cgi/pmidlookup?view=long&pmid=16549797" target="_blank">Publisher
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Petsch, Mylne, Botella (2005). Cosuppression of eukaryotic release factor 1-1 in

Arabidopsis affects cell elongation and radial cell division. Plant Physiology 139:

115-126. PubMed,
href="http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=16113224" target="_blank">Publisher
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Mylne, Greb, Lister and Dean (2004). Epigenetic Regulation in the Control of Flowering. in

Epigenetics, eds. Stillman & Stewart (CSHL Press, Cold Spring Harbor, New York), Vol. LXIX, pp. 457–464.

PubMed,
href="http://www.mylne.org/files/Mylne_2004_CSHL_69_457.pdf" target="_blank">PDF
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Bastow, Mylne, Lister, Lippman, Martienssen, Dean (2004). Vernalization requires epigenetic

silencing of FLC by histone methylation. Nature 427: 164-167.
href="http://www.ncbi.nlm.nih.gov/pubmed/14712277" target="_blank">PubMed
,
href="http://dx.doi.org/10.1038/nature02269" target="_blank">Publisher
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Boss, Bastow, Mylne, and Dean (2004). Multiple pathways in the decision to flower: enabling,

promoting, and resetting. Plant Cell 16: S18-31.
href="http://www.ncbi.nlm.nih.gov/pubmed/15037730" target="_blank">PubMed
,
href="http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=15037730" target="_blank">Publisher
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Levy, Mesnage, Mylne, Gendall, and Dean (2002). Multiple roles of Arabidopsis VRN1 in

vernalization and flowering time control. Science 297: 243-246 .
href="http://www.ncbi.nlm.nih.gov/pubmed/12114624" target="_blank">PubMed
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href="http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=12114624" target="_blank">Publisher
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Funding received
Australian Research Council LIEF Grant (2014) Biomolecular Interaction Facility. Bond, Vrielink, Filipovska, Mylne, Small, Mancera, Oliver.
Australian Research Council Discovery Project (2013-2015) A new and rapidly evolving class of plant peptides. Mylne, Panero & Schilling.
Australian Research Council Future Fellowship (2013-2016) Genetic evolution of plant proteins with biomedical applications. Mylne.
Australian Research Council Discovery Project (2012-2014) How do sunflowers make protein drugs in their seeds? Mylne, Rosengren & Suga.
UQ-UWA Bilateral Research Collaboration Award (2012) Exploring the genetic origin of a protein hijack event in daisies with next-generation sequencing. Mylne & Whelan.
National Health & Medical Research Council Project Grant (2011-2013) Harnessing plants to produce peptide drugs. Craik & Mylne.
Australian Research Council Discovery Project (2008-2012) Circular plant proteins with pharmaceutical applications. Mylne.
Memberships
2008-current: Member, Australian Society of Plant Scientists
2008-current: Member, Australian Society for Biochemistry and Molecular Biology
Honours and awards
2015: UWA Vice-Chancellor’s Mid-Career Research Award
2013: Feinberg Foundation Visiting Faculty Program Fellow, Department of Biological Chemistry, Weizmann Institute, Israel
2012: Australian Research Council Future Fellowship
2012: Peter Goldacre Award, awarded by Australian Society of Plant Scientists
2010: John S. Mattick Fellowship
2009: American Society of Plant Biologists Top Authors 2004-2008
2009: UQ Foundation Research Excellence Award
2008: Appointed Captain, Royal Aust. Army Medical Core, Australian Reserve Army
2007: Australian Research Council Queen Elizabeth II Fellowship
Previous positions
2013-2015: Honorary and Adjunct appointment, Institute for Molecular Bioscience (IMB), UQ, Brisbane, Australia
2010-2012: The John S. Mattick Fellow, IMB, UQ Brisbane, Australia
2008-2012: Australian Research Council Queen Elizabeth II (QEII) Fellow, Division of Chemistry & Structural Biology, Institute for Molecular Bioscience (IMB), UQ, Brisbane, Australia
2008-2012: Australian Army Specialist Service Officer, Research Scientist, Division of Drug Resistance & Diagnostics, Army Malaria Institute, Enoggera, Australia
2007: University of Queensland (UQ) Post-Doctoral Research Fellow, IMB, UQ, Brisbane, Australia
2006: Post-Doctoral Research Officer with Prof. David Craik, IMB, UQ, Brisbane, Australia
2001-2005: Project Scientist with Prof. Caroline Dean (FRS, NAS, OBE), Department of Cell & Developmental Biology, John Innes Centre, Norwich, England, UK
2001: Visiting Scientist with Victoriano Valpuesta, Dpto. de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Spain
1999-2000: Visiting Scientist with Phil Benfey, Department of Biology, New York University, Manhattan, New York, USA
Teaching
2015-now: BIOC2001 Biochemistry and Molecular Biology of the Cell, Course lecturer
2015-now: MSCI4006 Advanced Techniques in Molecular Sciences, Course lecturer
2015-now: SCIE4001 The Objectives and Applications of Genomics, Course lecturer
2015-now: GENE4001 Advanced Studies in Genetics and Genomics, Course lecturer
2015: MSCI4098 Seminar Appraisal and Presentation Part 1, Co-Coordinator
2015: MSCI4099 Seminar Appraisal and Presentation Part 2, Co-Coordinator
2014-now: BIOC3001 Molecular Biology, Course lecturer
2013-now: CHEM3001 Essential Chemical Skills, Guest lecturer
Current external positions
2013-2015: Adjunct Associate Professor, Institute for Molecular Bioscience (IMB), UQ, Brisbane, Australia
New and noteworthy
2015 May: Sunflower protein 'scissors' provide sunny news for medicine, UWA Press Release
2014 March: Ancient buried treasure found in in daisy seeds, UWA Press Release
2013 September: Moving genes have scientists seeing spots, UWA Press Release
Research profile
Research profile and publications
 

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