The University of Western Australia

UWA Staff Profile

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Josh Mylne

Assoc/Prof Joshua Mylne

Associate Professor
Molecular Sciences, School of

Contact details
School of Molecular Sciences
The University of Western Australia (M310)
35 Stirling Highway
+61 8 6488 4415
+61 8 6488 4446
Personal homepage
BSc PhD Qld
Dr Mylne (PhD, Botany) worked at the John Innes Centre (2001-2005), using molecular genetics to study proteins that accelerate flowering in response to prolonged cold (vernalization). In 2006 he moved to the Division of Chemistry & Structural Biology at The Institute for Molecular Bioscience (IMB, UQ) where he held a QEII Fellowship (2008-2012) and was the inaugural John S. Mattick Fellow (2010-2012). In 2013 he joined the faculty at The University of Western Australia and took up an ARC Future Fellowship in the School of Chemistry & Biochemistry and The ARC Centre of Excellence in Plant Energy Biology. His research interests are protein evolution, molecular mechanisms underlying the biosynthesis of bioactive peptides and increasing the discovery of novel herbicides and novel modes of action.
Key research
Plant peptide biosynthesis
Protease evolution
Fisher, Zhang, Taylor, Howard, Berkowitz, Debowski, Behsaz, Whelan, Pevzner, Mylne (2018) A family of small, cyclic peptides buried in preproalbumin since the Eocene epoch Plant Direct 2: e00042. Publisher.

Haywood, Schmidberger, James, Nonis, Sukhoverkov, Elias, Bond, Mylne (2018) Structural basis of ribosomal peptide macrocyclization in plants eLife 7: e32955. PubMed, Publisher.

Corral, Leroux, Tresch, Newton, Stubbs*, Mylne* (2018) A herbicide structure-activity analysis of the antimalarial lead compound MMV007978 against Arabidopsis thaliana Pest Manag. Sci. on-line: 30 January 2018. PubMed, Publisher.

Franke, Mylne, Rosengren (2018) Buried treasure: biosynthesis, structures and applications of cyclic peptides hidden in seed storage albumins Nat. Prod. Rep. on-line: 30 January 2018. PubMed, Publisher.

Wallace, Waraich, Debowski, Corral, Maxwell, Mylne*, Stubbs* (2018) Developing ciprofloxacin analogues against plant DNA gyrase: A novel herbicide mode of action Chem. Commun. on-line: 25 January 2018. Publisher.

Corral, Leroux, Tresch, Newton, Stubbs* Mylne* (2017) Exploiting the evolutionary relationship between malarial parasites and plants to develop new herbicides Angew. Chem. Int. Ed. Engl. on-line: early 27 June 2017. PubMed, Publisher.

Franke, James, Mobli, Colgrave, Mylne, Rosengren (2017) Two proteins for the price of one: structural studies of the dual-destiny Preproalbumin with Sunflower Trypsin Inhibitor-1 J. Biol. Chem. on-line: early 23 May 2017. PubMed, Publisher.

Corral, Leroux, Stubbs, Mylne (2017) Herbicidal properties of antimalarial drugs Scientific Reports 7: 45871. PubMed, Publisher.

Jayasena, Fisher, Panero, Secco, Bernath-Levin, Berkowitz, Taylor, Schilling, Whelan, Mylne (2017) Stepwise evolution of a buried inhibitor peptide over 45 million years Molecular Biology and Evolution 34: 1505-1516. PubMed, Publisher.

James, Jayasena, Zhang, Berkowitz, Secco, Knott, Whelan, Bond, Mylne (2017) Evidence for ancient origins of Bowman-Birk inhibitors from Selaginella moellendorffii Plant Cell 29: 461-473. PubMed, Publisher.

James, Haywood, Mylne* (2017) Macrocyclization by asparaginyl endopeptidases. New Phytologist on-line: early 21 March 2017. PubMed, Publisher.

Grage, Sani, Cheneval, Henriques, Schalck, Heinzmann, Mylne, Mykhailiuk, Afonin, Komarov, Separovic, Craik, Ulrich (2017) Orientation and location of the cyclotide kalata B1 in phospholipid bilayers revealed by solid-state NMR. Biophysical Journal 112: 630-642. PubMed, Publisher.

Elliott, Franke, Armstrong, Craik, Mylne*, Rosengren* (2017) Natural structural diversity within a conserved cyclic peptide scaffold Amino Acids 49: 103-116. PubMed, Publisher.

Franke, Jayasena, Fisher, Swedberg, Taylor, Mylne, Rosengren (2016) Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana) Biopolymers 106: 806-817. PubMed, Publisher

Franke, Colgrave, Mylne, Rosengren (2016) Mature forms of the major seed storage albumins in sunflower: a mass spectrometric approach Journal of Proteomics 147: 177-186. PubMed, Publisher

Evans-Roberts, Mitchenall, Wall, Leroux, Mylne, Maxwell (2016) DNA gyrase is the target for the quinolone drug ciprofloxacin in Arabidopsis thaliana J. Biol. Chem. 291: 3136-3144. PubMed, Publisher.

Jayasena, Franke, Rosengren, Mylne (2016) A tripartite approach identifies the major sunflower seed albumins Theoretical and Applied Genetics 129: 613-629. PubMed, Publisher.

Bernath-Levin, Nelson, Elliott, Jayasena, Millar, Craik, Mylne (2015) Peptide macrocyclization by a bifunctional endoprotease Chemistry & Biology 22: 571–582. PubMed, Publisher.

Gandy, Corral, Mylne*, Stubbs* (2015) An interactive database to explore herbicide physicochemical properties. Organic & Biomolecular Chemistry 13: 5586-5590. (*co-corresponding) PubMed, Publisher.

Mahatmanto, Mylne, Poth, Swedberg, Kaas, Schaefer, Craik (2015) The evolution of Momordica cyclic peptides. Molecular Biology and Evolution 32: 392-405. PubMed, Publisher.

Jayasena, Secco, Berkowitz, Bernath-Levin, Whelan, Mylne (2014) Next generation sequencing and de novo transcriptomics to study gene evolution. Plant Methods 10: 34. PubMed, Publisher.

Elliott, Delay, Liu, Phua, Rosengren, Benfield, Panero, Colgrave, Jayasena, Dunse, Anderson, Schilling, Ortiz-Barrientos, Craik, Mylne (2014) Evolutionary origins of a bioactive peptide buried within preproalbumin. Plant Cell 26: 981-995. PubMed, Publisher.

Mylne, Hara-Nishimura, Rosengren (2014) Seed storage albumins: biosynthesis, trafficking and structures (Goldacre Review). Functional Plant Biology on-line early. Publisher.

Mahatmanto, Poth, Mylne, Craik (2014) A comparative study of extraction methods reveals preferred solvents for cystine-knot peptide isolation from Momordica cochinchinensis seeds. Fitoterapia 95: 22-33. PubMed, Publisher.

Rosa*, De Lucia*, Mylne*, Zhu*, Ohmido, Pendle, Kato, Shaw, Dean (2013) Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization. Genes & Development 27: 1845-1850. (*equal) PubMed, Publisher.

King, Chanson, McCallum, Ohme-Takagi, Byriel, Hill, Martin, Mylne (2013) The Arabidopsis B3 domain protein VERNALIZATION1 is involved in processes essential for development with structural and mutational studies revealing its DNA binding surface. J. Biol. Chem. 288: 3198–3207. PubMed, Publisher.

Mylne, Chan, Chanson, Daly, Schaefer, Bailey, Nguyencong, Cascales, Craik (2012) Cyclic peptides arising by evolutionary parallelism via asparaginyl-endopeptidase mediated biosynthesis. Plant Cell 24: 2765-2778. PubMed, Publisher.

Poth, Mylne, Grassl, Lyons, Millar, Colgrave, Craik (2012) Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae). J. Biol. Chem. 287: 27033-27046. PubMed, Publisher.

Craik, Henriques, Mylne, Wang (2012) Cyclotide isolation, characterization and bioassay. in Natural product biosynthesis by microorganisms and plants (3rd edition) Methods in Enzymology 516: 37-62. PubMed, Publisher.

Craik, Swedberg, Mylne, Cemazar (2012) Cyclotides as a basis for drug design. Expert Opinion on Drug Discovery 7:179-194. PubMed, Publisher.

Mylne, Mas, Hill (2012) NMR assignment and secondary structure of the C-terminal DNA binding domain of Arabidopsis thaliana VERNALIZATION1. Biomolecular NMR Assignments 6: 5-8. PubMed, Publisher.

Mohimani, Liu, Mylne, Poth, Colgrave, Tran, Selsted, Dorrestein, Pevzner (2011) Cycloquest: Identification of cyclopeptides via database search of their mass spectra against genome databases. Journal of Proteome Research 10: 4505-4512. PubMed, Publisher.

Mylne & Wigge (2011) Florigen takes two to tango. Nature Chemical Biology 7:665-666. PubMed, Publisher.

Elliott, Craik, Mylne (2011) Sunflower trypsin inhibitor-1: chemical v. biological synthesis. Proceedings of the 22nd American Peptide Symposium, ed. Michal Lebl, Prompt Scientific Publishing, San Diego, CA, pp86-87. PDF.

Mylne, Colgrave, Daly, Chanson, McCallum, Jones, Elliott, Craik (2011) Albumins and their processing machinery are hijacked for cyclic peptides in sunflower. Nature Chemical Biology 7: 257-259. PubMed, Publisher.

Box, Coustham, Dean, Mylne (2011) Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis. Plant Methods 7: 7. PubMed, Publisher.

Mylne, Wang, van der Weerden, Craik (2010). Cyclotides are a component of the innate defence of Oldenlandia affinis. Biopolymers (Pept. Sci.) 94: 635-646. PubMed, Publisher.

Qin, McCallum, Kaas, Saska, Craik, Mylne (2010). Identification of candidates for cyclotide biosynthesis and cyclization by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics 11: 111. PubMed, Publisher.

Craik, Mylne, Daly (2010). Cyclotides: macrocyclic peptides with applications in drug design and agriculture. Cell. Mol. Life Sci. 67: 9-16. PubMed, Publisher.

Trabi, Mylne, Sando, Craik (2009). Circular proteins from Melicytus (Violaceae) refine the conserved protein and gene architecture of cyclotides. Organic & Biomolecular Chemistry. 7: 2378 – 2388. PubMed, Publisher.

King, Hill, Martin, Mylne (2009). Expression, purification and preliminary X-ray diffraction studies of VERNALIZATION1208-341 from Arabidopsis thaliana. Acta Crystallogr. F 65: 291-294. PubMed, Publisher.

Pien, Fleury, Mylne, Crevillen, Inzé, Avramova, Dean, Grossniklaus (2008). ARABIDOPSIS TRITHORAX1 dynamically regulates FLOWERING LOCUS C activation via histone 3 lysine 4 trimethylation. Plant Cell 20: 580-588. PubMed, Publisher.

Mylne & Craik (2008). 15N cyclotides by whole plant labeling. Biopolymers (Pept. Sci.) 90: 575-580. PubMed, Publisher.

Herrmann, Burman, Mylne, Karlsson, Gullbo, Craik, Clark, Göransson (2008). The alpine violet, Viola biflora, is a rich source of novel cyclotides with potent cytotoxicity. Phytochemistry 69: 939-952. PubMed, Publisher.

Greb*, Mylne*, Crevillen, Geraldo, An, Gendall, Dean (2007). The PHD finger protein VRN5 functions in the epigenetic silencing of Arabidopsis FLC. Current Biology 17: 73-78. (*equal) . PubMed, Publisher.

Mulvenna, Mylne, Bharathi, Burton, Shirley, Fincher, Anderson, Craik (2006). Discovery of cyclotide-like protein sequences in graminaceous crop plants: ancestral precursors of circular proteins? Plant Cell 18: 2134-2144. PubMed, Publisher.

Mylne, Barrett, Tessadori, Mesnage, Johnson, Bernatavichute, Jacobsen, Fransz, Dean (2006). LHP1, the Arabidopsis homologue of HETEROCHROMATIN PROTEIN1 is required for epigenetic silencing of FLC. Proc. Natl Acad. Sci. U.S.A. 103: 5012-5017. PubMed, Publisher.

Petsch, Mylne, Botella (2005). Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division. Plant Physiology 139: 115-126. PubMed, Publisher.

Mylne, Greb, Lister and Dean (2004). Epigenetic Regulation in the Control of Flowering. in Epigenetics, eds. Stillman & Stewart (CSHL Press, Cold Spring Harbor, New York), Vol. LXIX, pp. 457–464. PubMed, PDF.

Bastow, Mylne, Lister, Lippman, Martienssen, Dean (2004). Vernalization requires epigenetic silencing of FLC by histone methylation. Nature 427: 164-167. PubMed, Publisher.

Boss, Bastow, Mylne, and Dean (2004). Multiple pathways in the decision to flower: enabling, promoting, and resetting. Plant Cell 16: S18-31. PubMed, Publisher.

Levy, Mesnage, Mylne, Gendall, and Dean (2002). Multiple roles of Arabidopsis VRN1 in vernalization and flowering time control. Science 297: 243-246 . PubMed, Publisher.
Roles, responsibilities and expertise
2017-2019: Honours Coordinator (Biochemistry, Molecular Biology and Genetics), School of Molecular Sciences, UWA
Funding received
CSIRO Research+ Postdoctoral Fellowships (2017-2019) Generating novel RNA carrying peptides for control of agricultural diseases. Anderson, Singh, Mylne.
Australian Research Council Discovery Project (2016-2018) How scissors learn to glue: the catalysis of ligation by proteases. Mylne & Tawfik.
UWA Research Collaboration Award (2015) The structural enzymology behind a ligating protease. Mylne, Bernath-Levin.
Australian Research Council LIEF Grant (2014) Biomolecular Interaction Facility. Bond, Vrielink, Filipovska, Mylne, Small, Mancera, Oliver.
UQ-UWA Bilateral Research Collaboration Award (2014) How do sunflowers produce both a seed storage protein and a potent trypsin inhibitor from the same gene? Rosengren & Mylne.
Australian Research Council Discovery Project (2013-2015) A new and rapidly evolving class of plant peptides. Mylne, Panero & Schilling.
Australian Research Council Future Fellowship (2013-2016) Genetic evolution of plant proteins with biomedical applications. Mylne.
Australian Research Council Discovery Project (2012-2014) How do sunflowers make protein drugs in their seeds? Mylne, Rosengren & Suga.
UQ-UWA Bilateral Research Collaboration Award (2012) Exploring the genetic origin of a protein hijack event in daisies with next-generation sequencing. Mylne & Whelan.
National Health & Medical Research Council Project Grant (2011-2013) Harnessing plants to produce peptide drugs. Craik & Mylne.
Australian Research Council Discovery Project (2008-2012) Circular plant proteins with pharmaceutical applications. Mylne.
2008-current: Member, Australian Society of Plant Scientists
2008-current: Member, Australian Society for Biochemistry and Molecular Biology
Honours and awards
2017: Fulbright Professional Scholar, awarded by the Australian-American Fulbright Commission
2015: UWA Vice-Chancellor’s Mid-Career Research Award
2013: Feinberg Foundation Visiting Faculty Program Fellow, Department of Biological Chemistry, Weizmann Institute, Israel
2012: Australian Research Council Future Fellowship
2012: Peter Goldacre Award, awarded by Australian Society of Plant Scientists
2010: John S. Mattick Fellowship
2009: American Society of Plant Biologists Top Authors 2004-2008
2009: UQ Foundation Research Excellence Award
2008: Appointed Captain, Royal Aust. Army Medical Core, Australian Reserve Army
2007: Australian Research Council Queen Elizabeth II Fellowship
Previous positions
2017-2018: Senior Lecturer, School of Molecular Sciences, The University of Western Australia, Crawley, Perth, Australia
2012-2016: Australian Research Council Future Fellow, appointly jointly to the School of Chemistry & Biochemistry and the ARC Centre of Excellence in Plant Energy Biology
2013-2015: Honorary and Adjunct appointment, Institute for Molecular Bioscience (IMB), UQ, Brisbane, Australia
2010-2012: The John S. Mattick Fellow, IMB, UQ Brisbane, Australia
2008-2012: Australian Research Council Queen Elizabeth II (QEII) Fellow, Division of Chemistry & Structural Biology, Institute for Molecular Bioscience (IMB), UQ, Brisbane, Australia
2008-2012: Australian Army Specialist Service Officer, Research Scientist, Division of Drug Resistance & Diagnostics, Army Malaria Institute, Enoggera, Australia
2007: University of Queensland (UQ) Post-Doctoral Research Fellow, IMB, UQ, Brisbane, Australia
2006: Post-Doctoral Research Officer with Prof. David Craik, IMB, UQ, Brisbane, Australia
2001-2005: Project Scientist with Prof. Caroline Dean (FRS, NAS, OBE), Department of Cell & Developmental Biology, John Innes Centre, Norwich, England, UK
2001: Visiting Scientist with Victoriano Valpuesta, Dpto. de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Spain
1999-2000: Visiting Scientist with Phil Benfey, Department of Biology, New York University, Manhattan, New York, USA
2017-now: SCIE4481 Good, Bogus and Corrupted Science, Course lecturer
2017-now: GENE2230 Molecular Genetics, Course lecturer
2015-now: BIOC2001 Biochemistry and Molecular Biology of the Cell, Course lecturer
2015-now: MSCI4006 Advanced Techniques in Molecular Sciences, Course lecturer
2015-now: SCIE4001 The Objectives and Applications of Genomics, Course lecturer
2015-2017: GENE4001 Advanced Studies in Genetics and Genomics, Course lecturer
2015: MSCI4098 Seminar Appraisal and Presentation Part 1, Co-Coordinator
2015: MSCI4099 Seminar Appraisal and Presentation Part 2, Co-Coordinator
2014-now: BIOC3001 Molecular Biology, Course lecturer
2013-now: CHEM3001 Essential Chemical Skills, Guest lecturer
New and noteworthy
2018 March: Proteins date back to the time of sabre toothed cats, UWA Press Release
2018 February: Scientists reveal double life of sunflower enzyme, UWA Press Release
2017 December: Researchers from UWA awarded Fulbright scholarships, UWA Press Release
2017 June: Antimalarial drugs offer a smorgasbord of new herbicides, UWA Press Release
2017 April: UWA video release for World Malaria Day, UWA FaceBook
2017 April: Chemicals that cure malaria can kill weeds too, UWA Press Release
2017 March: Proteins hiding in proteins take an evolutionary shortcut, UWA Press Release
2017 March: Protein doppelgangers are long-lost cousins, UWA Press Release
2015 May: Sunflower protein 'scissors' provide sunny news for medicine, UWA Press Release
2014 March: Ancient buried treasure found in daisy seeds, UWA Press Release
2013 September: Moving genes have scientists seeing spots, UWA Press Release
Research profile
Research profile and publications

The University of Western Australia

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Last updated:
Tuesday, 3 November, 2015 2:39 PM