Asst/Prof Michael Clarke
- Contact details
The University of Western Australia (M010)
35 Stirling Highway
CRAWLEY WA 6009
- +61 8 6488 4457
- Room 3.42, Bayliss Building, Perth campus
- Michael Clarke's career initially involved 12 years working in clinical biochemistry in PathWest, culminating with a PhD studying antioxidants and health outcomes at UWA. In 2011 Michael joined UWA as a senior scientist in the Metabolomics Australia national network. Michael currently manages the Biological and Molecular Mass Spectrometry Facility (BMMSF) which also incorporates Metabolomics Australia within CMCA at UWA. Michaels' interests include hormone analysis using LCMS and drug pharmacokinetics in humans and animal models of disease.
In 2012 Michael established a reference assay for the analysis of Vitamin D in serum. The BMMSF is involved in several national projects studying Vitamin D and its effects on health outcomes in humans. The facility is recognised by the CDC for the analysis of Vitamin D in human serum and has recently passed certification for accuracy and precision for the 3rd year in a row.
- Key research
- Metabolomics in multiple sample types
- Vitamin D metabolism in humans
- Drug pharmacokinetics in a sheep model of human disease
- Vitamin E metabolism in humans
- Antioxidants in health and disease
- Method development in Mass Spectrometry
- John Molloy, Jennifer J. Koplin, Katrina J. Allen, Mimi L.K. Tang, Fiona Collier, John B. Carlin, Richard Saffery, David Burgner, Sarath Ranganathan, Terence Dwyer, Alister C. Ward, Margarita Moreno-Betancur, Michael Clarke, Anne Louise Ponsonby, Peter Vuillermin, The BIS investigator group
Vitamin D insufficiency in the first 6 months of infancy and challenge-proven IgE-mediated food allergy at 1 year of age: a case-cohort study
Allergy. 2017; Accepted manuscript online: 2 January DOI: 10.1111/all.13122
Matthew W Kemp, Masatoshi Saito, Haruo Usuda, Timothy J Molloy, Yuichiro Miura, Shinichi Sato, Shimpei Watanabe, Michael Clarke, Michael Fossler, Augusto Scmidt, Suhas G Kallapur, Boris W Kramer, John P Newnham, Alan H Jobe
Maternofetal pharmacokinetics and fetal lung responses in chronically catheterized sheep receiving constant, low-dose infusions of betamethasone phosphate
American Journal of Obstetrics and Gynecology. 2016; 215 (6), 775.
Matthew Kemp, Masatoshi Saito, Haruo Usuda, Yuichiro Miura, Shimepi Watanabe, Shinichi Sato, Michael Clarke, Michael Fossler, Augusto Schmidt, Suhas Kallapur, Boris Kramer, John Newnham, Alan Jobe
Low-Dose Steroid Infusions Achieve Lung Maturation in a Sheep Model of Pregnancy.
REPRODUCTIVE SCIENCES. 2016; 23, 192A-192A.
Crew RC, Mark PJ, Clarke MW, Waddell BJ
Obesity Disrupts the Rhythmic Profiles of Maternal and Fetal Progesterone in Rat Pregnancy
Bio Repord. 2016 Sep;95(3):55.
Wharfe MD, Mark PJ, Wyrwoll CS, Smith JT, Yap C, Clarke MW, Waddell BJ.
Pregnancy-induced adaptations of the central circadian clock and maternal glucocorticoids.
J Endocrinol. 2016 Mar;228(3):135-47.
Wu G, Johnson SK, Bornman JF, Bennett SJ, Clarke MW, Singh V, Fang Z.
Growth temperature and genotype both play important roles in sorghum grain phenolic composition.
Sci Rep. 2016 Feb 24;6:21835.
Black LJ, Anderson D, Clarke MW, Ponsonby A-L, Lucas RM, Ausimmune Investigator Group. Analytical Bias in the Measurement of Serum 25-Hydroxyvitamin D Concentrations Impairs Assessment of Vitamin D Status in Clinical and Research Settings. Ramagopalan SV, ed. PLoS ONE. 2015;10(8):e0135478. d
Ali A. Albarhania, Fiona Collier, Ronda F. Greavesa, Anne-Louise Ponsonby, Katrina J. Allen, Peter J. Vuillermin, Peter Roche, Michael W. Clarke.
Vitamins D and A can be successfully measured by LC–MS/MS in cord blood diluted plasma
Clinical Biochemistry. 2015 Nov;48(16-17):1105-12
de Busserolles F, Hart NS, Hunt DM, Davies WI, Marshall NJ, Clarke MW, Hahne D, Collin SP.
Spectral Tuning in the Eyes of Deep-Sea Lanternfishes (Myctophidae): A Novel Sexually Dimorphic Intra-Ocular Filter
Brain Behav Evol 2015;85:77-93
Inglis TJ, Hahne DR, Merritt AJ, Clarke MW.
Volatile sulphur compound profile distinguishes Burkholderia pseudomallei from Burkholderia thailandensis.
J Clin Microbiol. 2015 Jan 7, Part 2: JCM.03644-14
Kemp MW, Miura Y, Payne MS, Jobe AH, Kallapur SG, Saito M, Stock SJ, Spiller OB, Ireland DJ, Yaegashi N, Clarke M, Hahne D, Rodger J, Keelan JA, Newnham JP.
Maternal intravenous administration of azithromycin results in significant fetal uptake in a sheep model of second trimester pregnancy.
Antimicrob Agents Chemother. 2014 Nov;58(11):6581-91
Clarke, M.W, Tuckey, R.C, Gorman, S, Holt, B, Hart, P. Optimized 25-hydroxyvitamin D analysis using liquid–liquid extraction with 2D separation with LC/MS/MS detection, provides superior precision compared to conventional assays.
Metabolomics. 2013 March 1-10.
Michael W Clarke, Brian Cooke, Kirsten Hoad and Paul Glendenning
Improved Plasma Free Metadrenaline analysis requires MCX solid phase extraction prior to LCMS detection.
Annals of Clinical Biochemistry. 2011 Jul; 48(4): 352-7.
Thomas Ledowski, Elaine Pascoe, Bernice Ang, Torben Schmarbeck, Michael W Clarke, Vimal Kapoor.
Monitoring of intraoperative Nociception: Skin conductance and Surgical Stress Index versus Stress Hormone Plasma Levels.
Anaesthesia. 2010 Oct; 65(10): 1001-6.
Samarina Musaad, Ken Robertson, Michael W Clarke, Gary French, Weldon Chiu, John R. Burnett.
Extreme hyperalphalipoproteinaemia in a patient with a paraspinal lipoma
Ann Clin Biochem. 2010 Jan; 47(Pt 1): 90-3.
Wu JH, Hodgson JM, Clarke MW, Puddey IB, Croft KD.
Inhibition of 20-hydroxyeicosatetraenoic acid synthesis using specific plant lignans: in vitro and human studies.
Hypertension. 2009 Nov; 54(5): 1151-8.
Clarke MW, Ledowski T, Wu JH, Hodgson JM, Puddey IB, Burnett JR, Croft KD.
Vitamin E Supplementation and Hepatic Drug Metabolism in Humans
J Cardiovasc Pharmacol. 2009 Dec; 54(6): 491-6.
Clarke MW, Burnett JR, Croft KD.
Vitamin E Metabolism in Health and Disease.
Crit Rev Clin Lab Sci. 2008; 45(5): 417-50. Review.
Ward NC, Wu JH, Clarke MW, Puddey IB, Burke V, Croft KD, Hodgson JM.
The effect of vitamin E on blood pressure in individuals with type 2 diabetes: a randomized, double-blind, placebo-controlled trial.
J Hypertens 2007; 25: 227-34.
Clarke MW, Hooper AJ, Headlam HA, Wu JH, Croft KD, Burnett JR.
Assessment of tocopherol metabolism and oxidative stress in familial hypobetalipoproteinemia.
Clin Chem 2006; 52: 1339-45.
Clarke MW, Ward NC, Wu JH, Hodgson JM, Puddey IB, Croft KD.
Supplementation with mixed tocopherols increases serum and blood cell gamma-tocopherol but does not alter biomarkers of platelet activation in subjects with type 2 diabetes.
Am J Clin Nutr 2006; 83: 95-102.
Ledowski T, Paech MJ, Clarke MW, Schug SA.
The influence of catecholamines on pseudocholinesterase enzymatic activity. Results of a laboratory investigation.
J Clin Monit Comput. 2006; Oct; 20(5): 329-32.
Wu JH, Hodgson JM, Ward NC, Clarke MW, Puddey IB, Croft KD.
Nitration of gamma-tocopherol prevents its oxidative metabolism by HepG2 cells.
Free Radic Biol Med 2005; 39: 483-94.
John R Burnett and Michael W Clarke “Vitamin E” in Principles of Free Radical Biomedicine, Nova Science Publishers, Hauppauge, New York, USA, 2011
- Roles, responsibilities and expertise
- Technique group leader in Mass Spectrometry and Metabolomics
Research Fellow (Metabolomics Australia)
- Funding received
- Rural Industries Research & Development Corporation, 2016, $130,500
Development of honeybee products from a biodiversity hotspot
Katherine Hammer, Connie Locher, Michael Clarke (CI)
MS Research Australia, 2014, $180,000
Project Title: Vitamin D metabolites and risk of multiple sclerosis in the Ausimmune Study
Investigators: Robyn Lucas, Anne-Louise Ponsonby, Bruce Taylor, Lucinda Black, Michael Clarke(CI)
Telethon-Perth Children’s Hospital Research Fund 2014, $ $200,000
Project title: Improving gut biodiversity with soluble fibre in early life to prevent inflammatory disease – a pilot study to study effects on early immune development
Investigators: Susan Prescott, Desiree De Silva, Charles Mackay, Michael Clarke (CI), Rae-Chi Huang
National collaborative research infrastructure grant (NCRIS 2) 2013-14, $650,000
Bioplatforms Australia CRIS funding for Metabolomics
Investigators: Steven Smith, Michael Clarke (CI)
Bioplatforms Australia ex Education Investment Fund (EIF) 2011, $800,000
Investigators: Steven Smith, Michael Clarke (CI)
Royal Perth Hospital Medical Research Foundation Grant 2008, A$10K
Project Title: Correlation between Skin Conductance Monitoring, Surgical Stress Index and stress hormone plasma levels for assessment of intraoperative depth of analgesia
Investigators: Thomas Ledowski, Kevin Croft, Michael Clarke (CI)
- Member of the Metabolomics Society
Member of Australian Society of Medical Research
Member of Australasian Research Management Society
Member of the Australian and New Zealand Society for Mass Spectrometry (ANZSMS)
Member of the DOHaD Society of Australia and New Zealand
Member of the Endocrine Society of Australia
Member of Australian Institute of Tropical Health and Medicine
- Honours and awards
- Finalist at the Young Investigators Day at Royal Perth Hospital, October 2007
Finalist at the University of Western Australia Research Showcase, October 2007
First Prize Award for the Young Investigators Day at Royal Perth Hospital, August 2006 in the Clinical section
First Prize Award for Patient Orientated Research at the University of Western Australia Research Showcase, July 2006
- Previous positions
- Research Development Advisor
Faculty of Medicine, Dentistry and Health Sciences
University of Western Australia
Senior Medical Scientist
Core Chemical Pathology and Biochemistry
HPLC and Mass Spectrometry Laboratory
PathWest, Royal Perth Hospital
- Semester 2,2009
Curtin University of Technology
Teaching second year Clinical Biochemistry students with emphasis on laboratory techniques
- Useful links
- Current projects
- D-Light project, see http://telethonkids.org.au/d-light/
- Research profile
Research profile and publications