Assoc/Prof Nathan Pavlos
Orthopaedic Research Unit
- Contact details
- Orthopaedic Research Unit
The University of Western Australia (M508)
35 Stirling Highway
CRAWLEY WA 6009
- +61 8 6457 2083
- +61 8 6457 3210
- BSc PhD W.Aust.
- Dr Nathan Pavlos is an Associate Professor and Head of the Cellular Orthopaedics Laboratory, School of Biomedical Sciences at the University of Western Australia. He completed his undergraduate (1999) and PhD studies in Bone Cell Biology (2005) at UWA. In 2007 Dr Pavlos was awarded a NHMRC CJ Martin (Biomedical) Overseas Research Fellowship and carried out his postdoctoral training at the Max-Planck-Institute (MPI) for Biophysical Chemistry, Germany, from 2007-2009 with Prof Reinhard Jahn where he worked on the molecular regulation of synaptic vesicle exo-endocytosis in neurons. In 2010 Dr Pavlos returned to UWA to start up his own laboratory with a focus on intracellular trafficking and transport in bone cells.
- Key research
- Dr Pavlos’s current research interests focus on the molecular mechanisms of bone-resorbing osteoclasts, the major cellular protagonists underlying a number of highly debilitating musculoskeletal diseases including osteoporosis, osteoarthritis, Paget's disease and tumour-mediated bone loss. To achieve this, he combines innovative microscopy-based platforms together with high-through-put screens including integrative genomic, quantitative proteomics and phenomics to systematically decipher the core molecular machinery underlying bone resorption. Along with his focus on osteoclasts and bone biology, he also has a long-standing interest in the molecular regulation and dynamics of intracellular trafficking, a process essential for normal cellular function and is often perturbed in many different diseases including skeletal disorders, neurological disease, cancer, inflammation and pathogen invasion.
- i)Original Peer Reviewed Articles:
1.Zhu S, Chim SM, Cheng TS, Ang E, Ng B, Lin B, Tickner J, Xu HZ, Pavlos NJ*, and Xu J* Calmodulin interacts with Rab3D and modulates osteoclastic bone resorption, Scientific Reports, (In Press, Accepted 3 November) * co-corresponding author
2.Clairfeuille T, Mas C, Chan ASM, Yang Z, Tello-Lafoz M, Chandra M, Widagdo J, Kerr MC, Paul B, Mérida I, Rohan D. Teasdale RD, Pavlos NJ, Victor Anggono V, Collins BM. A molecular code for endosomal recycling of phosphorylated cargos by the SNX27-retromer complex, Nature Structural & Molecular Biology Sep 5. doi: 10.1038/nsmb.3290.
3.Fujiwara T, Ye S, Castro-Gomes T, Winchell CG, Andrews NW, Voth D, Varughese KL, Mackintosh SG, Feng Y, Pavlos NJ, Nakamura T, Manolagas SC, Zhao H Plekhm1 in complex with Def8 and Rab7 regulates lysosome positioning and bone resorption in osteoclasts and is important for bone homeostasis in mice, Journal of Clinical Investigation-Insight (In Press, Accepted 18 August).
4. Tiulpakov A, White CW, Abhayawardana RS, See HB, Chan AS, Seeber RM, Heng JI, Dedov I, Pavlos NJ, Pfleger KD. Mutations of vasopressin receptor 2 including novel L312S have differential effects on trafficking. Mol. Endocrinol. 2016 Jun 29:me20161002.
5.Chan, A.S.M, Clairfeuille, T., Landao-Bassonga,E., Kinna, G., Ng, P.Y., Loo, L.S., Cheng, T.S., Zheng, M.H., Hong, W., Teasdale, R.D., Collins, B.M and Pavlos, N.J, Sorting Nexin 27 couples PTHR trafficking to retromer for signal regulation in osteoblasts during bone growth, Mol. Biol. Cell, 2016 Apr 15;27(8):1367-82.
6.Ali N, Jurczyluk J, Shay G, Tnimov Z, Alexandrov K, Munoz MA, Skinner OP, Pavlos NJ, Rogers MJ, A highly sensitive prenylation assay reveals in vivo effects of bisphosphonate drug on the Rab prenylome of macrophages outside the skeleton, Small GTPases 2015 Oct 2;6(4):202-211.
7.Pavlos NJ,* Bionitti B*, Riedel D, Wenzel D, Vorbrüggen G, Schalk A, Kühnel K, Boyken J, Erck C, Martens H, Chua JJE and Jahn R. The GTPase Rab26 links synaptic vesicles to the autophagy pathway, eLife 2015 Feb 2;4:e05597. (2015) (*equal contributor)
8.Lim WH, Wong G, Lim EM, Byrnes E, Zhu K, Devine A, Pavlos NJ, Prince RL, Lewis JR. Circulating Lipocalin 2 Levels Predict Fracture-Related Hospitalizations in Elderly Women: A Prospective Cohort Study, Journal of Bone and Mineral Research (2015 May 1. doi: 10.1002/jbmr.2546. [Epub ahead of print] (JBMR® Highlights Article May 2015)
9.Ng PY, Cheng TS, Zhao H, Ye S, Ang ESM, Khor EC, Feng HT, Xu J, Zheng MH and Pavlos NJ#. Disruption of the dynein-dynactin complex unveils motor specific functions in osteoclast formation and bone resorption. Journal of Bone and Mineral Research (2013) (Cover Page for 28:1 of Jan 2013)
10.Pavlos NJ# and Ng PY, “Fusion and Fission” unveils remarkable insights into osteoclast plasticity, DOI: 10.1007/s00223-012-9620-7 Calcified Tissue International (Accepted Jun 11), (2012) # Corresponding Author
11.Chua JJE , Butkevich E, Worseck JM, Kittelmann M, Grønborg M, Behrmann E, Stelzl U, Pavlos NJ, Lalowski MM, Eimer S, Wanker EE, Klopfenstein DR, Jahn R. A Kinesin-1 adaptor mediates phosphorylation-dependent axonal transport of Syntaxin 1. PNAS (Accepted March 5) (2012)
13.Ye S, Fowler TW, Pavlos NJ, Ng PY, Feng Y, Zheng MH, Manolagas SC and Zhao H. LIS1 Regulates Osteoclast Formation and Function through Its Interactions with Dynein/Dynactin and Plekhm1 PLoS One (Accepted Oct 13) (2011)
14.Pavlos NJ, Cheng TS, Qin A, Ng PY, Feng HT, Ang ES, Carello A, Sung CH, Jahn R, Zheng MH and Xu J. Tctex-1, a Novel Interaction Partner of Rab3d, Is Required for Osteoclastic Bone Resorption. Mol Cell Biol 31(7):1551-64. Epub Jan 24 (2011)
15.Pavlos NJ, Gronborg M, Riedel D, Chua JJ, Boyken J, Kloepper TH, Urlaub H, Rizzoli SO and Jahn R.. Quantitative analysis of synaptic vesicle Rabs uncovers distinct yet overlapping roles for Rab3a and Rab27b in Ca2+-triggered exocytosis. J Neurosci 30:13441-53 (2010)
16.Grønborg M, Pavlos NJ, Brunk I, Chua JE, Münster- Wandowski, A, Riedel D, Ahnert-Hilger G, Urlaub H and Reinhard Jahn, Quantitative comparison of glutamatergic and GABAergic synaptic vesicles unveils selectivity for few proteins including MAL2, a novel synaptic vesicle protein J Neurosci 6;30(1):2-12 (2010)
17.Feng H*, Cheng T*, Steer JH, Joyce DA, Pavlos NJ, Leong C, Kular J, Liu J, Feng X, Zheng MH, Xu J Myocyte enhancer factor 2 and microphthalmia-associated transcription factor cooperate with NFATc1 to transactivate the V-ATPase d2 promoter during RANKL-induced osteoclastogenesis. J Biol Chem 284:14667-14676 (2009) (Equal contribution)
18.Pavlos NJ*, Cheng T*, Wang C, Tan JW, Lin JM, Cornish J, Zheng MH, Xu J. Mutations within the TNF-like core domain of RANKL impair osteoclast differentiation and activation. Mol Endocrinol 23:35-46 (2009) (*Equal contribution)
19.Feng H*, Cheng T*, Pavlos NJ, Yip KH, Carrello A, Seeber R, Eidne K, Zheng MH, Xu J. Cytoplasmic terminus of vacuolar type proton pump accessory subunit Ac45 is required for proper interaction with V(0) domain subunits and efficient osteoclastic bone resorption. J Biol Chem 283:13194-13204 (2008) (Equal contribution)
20.Pavlos NJ, Xu J, Riedel D, Yeoh SG, Teitelbaum SL, Papadimitriou JM, Jahn R, Ross FP, Zheng MH. Rab3D regulates a novel vesicular trafficking pathway that is required for osteoclastic bone resorption. Mol Cell Biol 25(12):5253-69 (2005)
1.Pavlos NJ and Friedman PA, GPCR Signalling and Trafficking: The Long and the Short of it (Invited Review, Accepted Oct 23, Trends in Endocrinology and Metabolism)
- Roles, responsibilities and expertise
- Dr Pavlos is Treasurer for the Australian & and New Zealand Society for Orthopaedic Research and Treasurer for The Australian & New Zealand Orthopaedic Research Society. He is also an active reviewer for the National Health & Medical Research Council (NHMRC), Editorial Board Member for the Journal of Orthopaedic Translation and reviewer for several scientific journals
- Funding received
- NHMRC-Project Grants
NHMRC-CJ Martin (Overseas) Training Fellowship
Department of Health WA-NIRISA
Ramaciotti Establishment Grant-Perpetual Funding
The CASS Foundation
Sir Charles Gairder Group-Research Grants
UWA Research Grants Scheme
- Australian & New Zealand Orthopaedic Research Society (ANZORS)
Australian & New Zealand Bone and Mineral Society (ANZBMS)
American Society for Cell Biology (ASCB)
American Society for Bone and Mineral Research (ASBMR)
- Honours and awards
- 2011-New Independent Researcher Infrastructure Support Award (NIRIS) Award
2011-Sir Charles Gairdner Group Research Foundation Best Poster Award
2011-The CASS (Contributing to Australian Scholarship and Science) Foundation Travel Award
2010-Australian and New Zealand Bone and Mineral Society (ANZBMS)Travel Award
2007-American Society for Bone and Mineral Research (ASBMR) Travel Award- For Oral Presentation
2007-International Chinese Hard Tissue Society Young Investigator Prize- For best publication
2006-Raine Research Prize- For best publication
2006-Sol Posen Research Award (ANZBMS)- For best publication in the field of bone and mineral research
2006-University of Western Australia Graduate Research School Publication Prize in Biological Sciences
2002-Christopher & Margie Nordin Young Investigator Award (ANZBMS)- For best poster
2002-Australian and New Zealand Orthopaedic Research Society (ANZORS) Student Presentation Award
2001-Australian and New Zealand Orthopaedic Research Society (ANZORS) Travel Award.
2001-Australian and New Zealand Society for Cell and Developmental Biology Incorporated (ANZSCDBI) Prize-For best poster
2000-Roger Merlick Young Investigator Award (ANZBMS)- For best oral presentation
2000-Australian and New Zealand Bone and Mineral Society Travel Award
1999-Pfizer Prize, For best oral presentation
- Previous positions
- 2007-2009 NHMRC CJ Martin Fellow- Max-Planck-Institute for Biophysical Chemistry, Goettingen, Germany
State Representative (WA), Australian and New Zealand Society for Cell and Developmental Biology (ANZSCDB)(2013-2015)
- Path2210: Fundamentals of Pathology and Laboratory Medicine
Path 3308-Pathology and Laboratory Medicine II
Unit Coordinator (2010-2015)
SMED4221 Research Foundations Unit (MD Scholarly Activity)
Supervision: Principal and co-supervisor of successfully completed Honours, Masters and PhD students.
- Current external positions
- Treasurer- Australian & New Zealand Bone and Mineral Society
Treasurer- Australian & New Zealand Orthopaedic Research Society
- New and noteworthy
- 2013 ASSCR and Questacon Regenerative Medicine Image Competition: ‘Small Objects, Big Impact’ Award-Winning Image for presentation in the Stem Cell Stories Exhibition, Questacon, Canberra (2013); and the Royal Institute of Australia (Adelaide; Jun 2014-Jul 2014), - the closing credits of the Adelaide ABC 7:30 Report (July 2014), 2015 Stem Cell Stories Exhibition, SciTech in Perth (Apr-Sep) and 2015 Vivid Festival of ideas and images.
- Current projects
- Sorting-Out the Skeleton: The role of intracellular trafficking in bone homeostasis and disease.
- FOR 110314, SEO 920116
- Research profile
Research profile and publications