The University of Western Australia

UWA Staff Profile

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Livia Hool

Assoc/Prof Livia Hool

Research Associate Professor
Human Sciences, School of

Contact details
School of Human Sciences
The University of Western Australia (M311)
35 Stirling Highway
+61 8 6488 3307
+61 8 6488 1025
Room 1.96, Physiology Building, Perth campus
BSc PhD Macq.
* Gaston Bauer Cardiovascular Research Fellow, Cellular Electrophysiology Laboratory, Royal North Shore Hospital, Sydney 1991-1995
* American Heart Association Postdoctoral Fellow, Case Western Reserve University Cleveland Ohio, USA 1995-1997
* NHMRC Peter Doherty Fellow (Established the Cardiovascular Electrophysiology Laboratory) Physiology, The University of Western Australia 1998-2002
* NHMRC Career Development Award Fellow 2004 –2009
* ARC Future Fellow and Honorary NHMRC Senior Research Fellow 2011 - 2014
* NHMRC Senior Research Fellow 2015
Key research
1. Regulation of L-type Ca2+ channel function under pathological conditions such as hypoxia and oxidative stress
2. Regulation of the L-type Ca2+ channel by adrenergic receptor stimulation during hypoxia and oxidative stress
3. Regulation of mitochondrial function by the L-type calcium channel and the role of the cytoskeleton
4. Targeting the L-type Ca2+ channel to prevent the development of cardiomyopathy
5. Use of nanoparticle technology to improve efficacy of therapy
6. Use of CRISPR-like technology to prevent hypertrophy
7. Improving efficacy of uptake of oligomers to treat Duchenne Muscular Dystrophy cardiomyopathy
(Select publications)
Hool, L.C. Hypoxia increases the sensitivity of the L-type Ca2+ channel to b-adrenergic receptor stimulation via a C2 region-containing protein kinase C isoform, Circulation Research, 87 (12): pp 1164-1171 (2000)[Impact factor 11.8]

Hool, L.C. Hypoxia alters the sensitivity of the L-type Ca2+ channel tob-adrenergic receptor stimulation in the presence of a-adrenergic receptor stimulation, Circulation Research, 88 (10): pp 1036-1043 (2001)[Impact factor 11.8]

Hool, L.C., Arthur, P.G. Decreasing cellular hydrogen peroxide with catalase mimics the effects of hypoxia on the sensitivity of the L-type Ca2+ channel to b-adrenergic receptor stimulation in cardiac myocytes, Circulation Research, 91: pp 601-609 (2002)[Impact factor 11.8]

Hool, L.C. Differential regulation of the slow and rapid components of guinea-pig cardiac delayed rectifier K+ channels by hypoxia, The Journal of Physiology, 554.3: pp 743-754 (2004)

Hool, L.C., Di Maria C.A., Viola H.M., Arthur P.G. Role of NAD(P)H-oxidase in the regulation of cardiac L-type Ca2+ channel function during acute hypoxia, Cardiovascular Research, 67: pp 624-635 (2005) (Editorial on the paper published in the same issue pages 578-580).

Viola, H. M., Arthur, P.G., Hool, L.C. A transient exposure to hydrogen peroxide causes an increase in mitochondrial-derived superoxide as a result of sustained alteration in L-type Ca2+ channel function in the absence of apoptosis in adult ventricular myocytes, Circulation Research, 100: pp 1036-1044 (2007)[Impact factor 11.8]

Hool, L.C., Corry, B. Redox control of calcium channels: from mechanisms to therapeutic opportunities, Antioxidants & Redox Signaling, 9 (4): pp 409-435 (2007)

Kloda, A., Petrov, E., Myer, G.R., Nguyen, T., Hurst, A., Hool, L.C., Martinac, B. Molecules in Focus. Mechanosensitive channels of large conductance, International Journal of Biochemistry and Cell Biology 40: pp 164-69 (2008)

Hurst, A.C., Petrov, E., Kloda, A., Nguyen, T., Hool, L.C., Martinac, B. Molecules in Focus. MscS, the bacterial mechanosensitive channel of small conductance, International Journal of Biochemistry and Cell Biology, 40: pp 581-5 (2008)

Seenarain V, Viola HM, Ravenscroft G, Casey T, Lipscombe R, Ingley E, Laing N, Bringans S and Hool LC. Evidence for altered guinea pig ventricular cardiomyocyte protein expression and growth in response to 5 minute in vitro exposure to H2O2. J Proteome Research 9(4): 1985-94, 2010 [Impact factor 6]

Viola HM and Hool LC. Qo site of mitochondrial complex III is the source of increased superoxide after transient exposure to hydrogen peroxide. Journal of Molecular and Cellular Cardiology 49(5):875-885, 2010. [Impact factor 5.5] Cited on Faculty of 1000 (F1000 Prime).

Thomas MG, Stone, L, Evill L, Ong S, Ziman MR, Hool L. Bone marrow stromal cells as replacement cells for Parkinson's disease: generation of an anatomical but not functional neuronal phenotype. Translational Research 157(2):56-63, 2011 [Impact factor 2.9]

Tang H, Viola HM, Filipovska A, Hool LC. Cav1.2 calcium channel is glutathionylated during oxidative stress in guinea-pig and ischemic human heart Free Radical Biology and Medicine Oct 15;51(8):1501-11, 2011 [Impact Factor 5.7]

Cook NL, Viola HM, Sharov VS, Hool LC, Schöneich C, Davies MJ. Myeloperoxidase-derived oxidants inhibit sarco/endoplasmic reticulum Ca2+-ATPase activity, and perturb Ca2+ homeostasis in human coronary artery endothelial cells Free Radical Biology and Medicine 52: 951-61, 2012 [Impact Factor 5.7].

Holobotovskyy V, Manzur M, Burchell J, Bolitho E, Tare M, Viola H, Hool L, Arnolda L, McKitrick D and Ganss R. Regulator of G protein signaling 5 regulates blood pressure homeostasis and vessel wall remodeling Circulation Research 112(5):781-791, 2013 [Impact factor 11.8]

Viola HM, Davies SKM, Filipovska A and Hool LC. L-type Ca2+ channel contributes to alterations in mitochondrial calcium handling in the mdx ventricular myocyte American Journal of Physiology 304(6):H767-75, 2013 [Impact Factor 4.0].

Clemons TD, Viola HM, House M, Swaminathan Iyer K and Hool L. Examining Efficacy of "TAT-less" Delivery of a Peptide against the L-Type Calcium Channel in Cardiac Ischemia-Reperfusion Injury ACS Nano 7(3): 2212-2220, 2013. [IF 12.08].

James E, Viola H, Hool L, Eggers P, Raston C and Boulos R. A novel antimicrobial agent reduces oxidative stress in cells RSC Advances 3: 7277-7281, 2013 [Impact Factor 5.4]

Hool LC. How does the heart sense changes in oxygen tension – a role for ion channels? Antioxidants and Redox Signaling in press accepted March 29, 2014 [IF 7.7]

Viola HM, Jordan MC, Roos KP and Hool LC. Decreased myocardial injury and improved contractility after administration of a peptide derived against the alpha-interacting domain of the L-type calcium channel JAHA 3(3): e000961, 2014

Viola HM, Adams A, Davies SKM, Fletcher S, Filipovska A and Hool LC. Impaired functional communication between the L-type calcium channel and mitochondria contributes to metabolic inhibition in the mdx heart PNAS 111(28): E2905-E2914, 2014 [Impact factor 9.9]. Selected for F1000Prime recommended as being of special significance in the field.

Clancy CE, Chen-Izu Y, Bers DM, Belardinelli L, Boyden PA, Csernoch L, Despa S, Fermini B, Hool LC, Izu L, Kass RS, Lederer WJ, Louch WE, Maack C, Matiazzi A, Qu Z, Rajamani S, Rippinger CM, Sejersted OM, O’Rourke B, Weiss JN, Varro A and Zaza A. Deranged sodium to sudden death Journal of Physiology (London) in press accepted November 14, 2014 IF 4.5

Tara R. Richman1, Judith A. Ermer1, Stefan M.K. Davies1, Kara L. Perks1, Helena M. Viola2, Anne-Marie J. Shearwood 1, Livia C. Hool2, Oliver Rackham1, 3 and Aleksandra Filipovska1Mutation in a mitochondria specific ribosomal protein compromises protein synthesis and liver function PLOS Genetics IF 8.2 in press accepted Feb 24 2015

Kular J, Tickner J, Pavlos N, Viola HM, Abel T, Lim BS, Yang X, Chen H, Cook R, Hool LC, Zheng MH and Xu J. Choline Kinase Beta Mutant Mice Exhibit Reduced Phosphocholine, Elevated Osteoclast Activity and Low Bone Mass Journal of Biological Chemistry in press accepted Dec 2, 2014 [Impact factor 4.6]

Hardy N, Viola HM, Johnstone VPA, Clemons TD, Cserne Szappanos H, Singh R, Smith NM, Swaminathan Iyer K and Hool LC. Nanoparticle Mediated Dual-delivery Enables Simultaneous Action Against Myocardial Damage and Oxidative Stress in Cardiac Ischemia- Reperfusion Injury ACS Nano 9(1): 279-289, 2015 IF 12.03

Grehl S, Viola HM, Fuller P, Carter K, Dunlop S, Hool L, Sherrard R and Rodger J Cellular and molecular changes to cortical neurons following low intensity repetitive magnetic stimulation at different frequencies Brain Stimulation 8(1): 114-123 2015 IF 4.5.

Challenor M, Doig R, Fuller P, Giacci M, Wale C, Hool L, Dunlop S, Swaminathan Iyer K, Rodger J and Fitzgerald M. Prolonged glutamate excitotoxicity increases GluR1 immunoreactivity but decreases mRNA of GluR1 and associated regulatory proteins in dissociated rat retinae in vitro Biochimie revision in review Feb 2015 IF 3.1

Funding received
NHMRC Project Grant 2006-2008
NHMRC Project Grant 2009-2011
NHMRC Project Grant 2010-2012
NHMRC Project Grant 2011-2013
NHMRC Project Grant 2013-2015
NHMRC Project Grant 2014-2016
NHMRC National Heart Foundation Biomedical Scholarship and Research Fellowship
ARC Future Fellowship
President ISHR (Australasian Section) 2014-16
Statutory member ISHR World Council 2014-16
Fellow American Heart Association
Fellow Cardiac Society of Australia and New Zealand
PCT Application (WO2013/113060). Use of the AID-TAT peptide in reduction of ischemia reperfusion injury
PHYL3300 Mammalian Cell Biology
PHYL3350 Physiological Control Mechanisms
PHYL3002 Physiology of Cardiovascular and Respiratory Systems
Current projects
PhD Students

- Stephanie Grehl - Use of rTMS in neurons (Co-supervised with Assoc Prof Jenny Rodger)
- Elizabeth Johnstone - G protein coupled receptors and disease (Co-supervised with Assoc Prof Kevin Pfleger)
- Padmapriya Muralidharan - Role of cysteines in redox modification of L type calcium channel function (Co-supervised with Assoc Prof Evan Ingley)

Postdoctoral staff
Dr Helena Viola
Dr Henrietta Cserne Szappanos
Dr Vicky Johnstone

Honours students/BPhil students
Mr Saiuj Bhat
Mr James Constantine
Mr Ashay Shah
Ms Emma Lu

Potential Student Projects

-Which cysteines on the L-type calcium channel are modified by reactive oxygen species? (Hons/PhD level)
- Improving efficacy of AID peptide in treatment of familial hypertrophic cardiomyopathy
- Improving efficacy of uptake of oligomers in treatment of Duchenne Muscular Dystrophy cardiomyopathy

-Professor Yoram Rudy, Washington University, St. Louis, Missouri USA
-Professor Boris Martinac, Victor Chang Cardiac Research Institute Sydney
-Professor Christopher Semsarian, Centenary Institute Sydney
-Professors Kricket Seidman and Jon Seidman, Harvard University, USA
-Professor Swaminatha Iyer, Chemistry and Biochemistry, UWA
-Professor Nigel Laing, The Harry Perkins Institute for Medical Research
-Professor Aleksandra Filipovska, The Harry Perkins Institute for Medical Research
-Assoc Prof Ben Corry, ANU
-Assoc Prof Evan Ingley, The Harry Perkins Institute for Medical Research
- Professor Ken Roos, University of California Los Angeles
- Dr Pilar Blancafort The Harry Perkins Institute for Medical Research
- Professors Sue Fletcher and Steve Wilton, Murdoch University
- Professor Susan Hamilton Baylor Medical Center Houston Texas
Research profile
Research profile and publications

The University of Western Australia

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Last updated:
Tuesday, 3 November, 2015 2:39 PM